Chen B Y, Wang Y, Janes H W
Department of Plant Science, Rutgers University, New Brunswick, New Jersey 08903, USA.
Plant Physiol. 1998 Jan;116(1):101-6. doi: 10.1104/pp.116.1.101.
The intracellular location of ADP-glucose pyrophosphorylase (AGP) in developing pericarp of tomato (Lycopersicon esculentum Mill) has been investigated by immunolocalization. With the use of a highly specific anti-tomato fruit AGP antibody, the enzyme was localized in cytoplasm as well as plastids at both the light and electron microscope levels. The immunogold particles in plastids were localized in the stroma and at the surface of the starch granule, whereas those in the cytoplasm occurred in cluster-like patterns. Contrary to the fruit, the labeling in tomato leaf cells occurred exclusively in the chloroplasts. These data demonstrate that AGP is localized to both the cytoplasm and plastids in developing pericarp cells of tomato.
通过免疫定位研究了ADP - 葡萄糖焦磷酸化酶(AGP)在番茄(Lycopersicon esculentum Mill)发育中的果皮细胞内的定位。使用高度特异性的抗番茄果实AGP抗体,在光学显微镜和电子显微镜水平上,该酶定位于细胞质以及质体中。质体中的免疫金颗粒定位于基质和淀粉粒表面,而细胞质中的免疫金颗粒呈簇状分布。与果实不同,番茄叶片细胞中的标记仅出现在叶绿体中。这些数据表明,AGP定位于番茄发育中的果皮细胞的细胞质和质体中。