Komarova Elena, Aldissi Matt, Bogomolova Anastasia
Fractal Systems Inc., 200 9th Avenue North, Ste. 100, Safety Harbor, FL 34695, USA.
Biosens Bioelectron. 2005 Jul 15;21(1):182-9. doi: 10.1016/j.bios.2004.07.025.
A novel reagentless direct electrochemical DNA sensor has been developed using ultrathin films of the conducting polymer polypyrrole doped with an oligonucleotide probe. Our goal was to develop a prototype electrochemical DNA sensor for detection of a biowarfare pathogen, variola major virus. The sensor has been optimized for higher specificity and sensitivity. It was possible to detect 1.6 fmol of complementary oligonucleotide target in 0.1 ml in seconds by using chronoamperometry. The sensitivity of the developed sensor is comparable to indirect electrochemical DNA sensors, which use electrochemical labels and reagent-intensive amplification. The developed sensing electrode is reusable, highly stable and suitable for storage in solution or in dry state.
一种新型的无试剂直接电化学DNA传感器已被开发出来，它使用了掺杂有寡核苷酸探针的导电聚合物聚吡咯超薄膜。我们的目标是开发一种用于检测生物战病原体——天花病毒的电化学DNA传感器原型。该传感器已针对更高的特异性和灵敏度进行了优化。通过计时电流法，能够在数秒内检测出0.1毫升中1.6飞摩尔的互补寡核苷酸靶标。所开发传感器的灵敏度与间接电化学DNA传感器相当，间接电化学DNA传感器使用电化学标记和试剂密集型扩增。所开发的传感电极可重复使用、高度稳定，适合在溶液中或干燥状态下储存。
