结构色半薄软骨切片的透射光显微镜检查。

Transmission light microscopy of structurally colored semithin cartilage sections.

作者信息

Malinin G I, Lo H K, Malinin T I

机构信息

Department of Physics, Georgetown University, Washington, DC 20057-0995, USA.

出版信息

Histochem Cell Biol. 1998 Jan;109(1):1-10. doi: 10.1007/s004180050196.

DOI:10.1007/s004180050196

PMID:9452950

Abstract

Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

摘要

通过三个串联反应，已实现将渗透化气管软骨成分转化为一系列层状干涉光栅。在二甲基亚砜中用乙酸酐氧化半薄的、LR白色包埋的软骨切片是转化过程的第一步。氧化软骨吡喃糖和呋喃糖与硫代碳酰肼的后续加成反应构成第二步。硫代碳酰腙还原蛋白银并随之用银光栅覆盖软骨成分，完成了将软骨切片转化为分层干涉滤光片系统的过程。在透射光下，转化软骨的所有成分都呈现出鲜艳的结构色，这使得能够对具有结构色的细胞和细胞外软骨成分进行详细的显微镜分析。