Secretory Expression and Purification of Respiratory Syncytial Virus G and F Ectodomain in Human Cells.

Respiratory syncytial virus (RSV) is one of the leading causes of respiratory viral infections in infants, immunocompromised individuals, and the elderly. The important role RSV F and G proteins play in disease progression and their ability to induce neutralizing antibodies make them desirable targets for vaccine production. Access to these expressed and purified proteins is also important to RSV research and diagnostics. Here, we describe methods to produce recombinant, secretory RSV F and G ectodomain in human cells and purify these proteins using Ni-Sepharose affinity chromatography.