Micheel B, Fiebach H, Wunderlich V
Arch Geschwulstforsch. 1979;49(6):485-9.
The present paper describes a competitive immunoassay using antiserum to FeLV p 27 and SSV-conjugated Sepharose beads. The assay is applied to compare the interspecies-specific antigenic determinants of the major structural proteins of type-C viruses of different mammalian species. The test proves to be highly sensitive and specific and may be used for the demonstration of viral proteins in crude cellular extracts.
本文描述了一种使用抗FeLV p 27抗血清和SSV偶联琼脂糖珠的竞争性免疫测定法。该测定法用于比较不同哺乳动物物种C型病毒主要结构蛋白的种间特异性抗原决定簇。该测试被证明具有高度敏感性和特异性,可用于在粗细胞提取物中检测病毒蛋白。