Uckert W, Wunderlich V, Bender E, Sydow G, Bierwolf D
Arch Virol. 1980;64(2):155-66. doi: 10.1007/BF01318019.
The protein pattern of a type-D retrovirus (PMFV) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Staining with Coomassie blue and labeling with 14C-leucine/14C-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. The 68,000 D-protein was shown to be a glycoprotein by incorporation of 3H-glucosamine and the 15,000 D-protein was identified as a phosphoprotein. By comparing PMFV with the closely related Mason-Pfizer monkey virus (MPMV) in co-electrophoresis experiments no clear difference was detected in viral 14C-leucine/14C-lysine profiles. The viruses differ, however, with respect to their glycoprotein patterns. A glycoprotein corresponding to the gp20 of MPMV has not been detected in PMFV irrespective of the cell line used for propagation of viruses.
利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对从人细胞系中分离并在其中增殖的D型逆转录病毒（PMFV）的蛋白质模式进行了研究。考马斯亮蓝染色和用¹⁴C-亮氨酸/¹⁴C-赖氨酸标记显示出五条病毒多肽，其分子量分别为10,000、12,000、15,000、25,000和68,000。通过掺入³H-葡糖胺表明68,000 D蛋白是一种糖蛋白，15,000 D蛋白被鉴定为磷蛋白。在共电泳实验中，通过将PMFV与密切相关的梅森-辉瑞猴病毒（MPMV）进行比较，未检测到病毒¹⁴C-亮氨酸/¹⁴C-赖氨酸图谱有明显差异。然而，这两种病毒在糖蛋白模式方面有所不同。无论用于病毒增殖的细胞系如何，在PMFV中均未检测到与MPMV的gp20相对应的糖蛋白。
