Stalcup R P, Baric R S, Leibowitz J L
Department of Pathology and Laboratory Medicine, Texas A&M University College of Medicine, 208 Reynolds Building, College Station, Texas, 77843-1114, USA.
Virology. 1998 Feb 1;241(1):112-21. doi: 10.1006/viro.1997.8957.
Temperature-sensitive (ts) mutant viruses have been useful for the study of replication processes in many viral systems. To determine how our panel of MHV-JHM-derived RNA- ts mutants (Robb et al., 1979) is genetically related to other panels of MHV RNA- ts mutants, we tested our mutants for complementation with representatives from two different sets of MHV-A59 ts mutants (Koolen et al., 1983; Schaad et al., 1990). These three ts mutant panels together comprise eight genetically distinct complementation groups. Considerable genetic similarity was observed among the three mutant panels. Only three complementation classes are unique to their particular mutant panel, and genetically equivalent mutants were not observed within the other two mutant panels. There are two overlapping complementation groups between the mutant sets derived from MHV-A59 and four overlapping complementation classes between the MHV-JHM panel and the MHV-A59 panels. Two complementation groups had representative mutants in all three mutant panels. One of these latter complementation classes demonstrated nonreciprocal complementation patterns consistent with intragenic complementation.
温度敏感（ts）突变病毒已被用于许多病毒系统中复制过程的研究。为了确定我们的一组源自MHV-JHM的RNA-ts突变体（Robb等人，1979年）与其他几组MHV RNA-ts突变体在遗传上的关系，我们测试了我们的突变体与来自两组不同的MHV-A59 ts突变体（Koolen等人，1983年；Schaad等人，1990年）的代表的互补作用。这三组ts突变体共同构成了八个遗传上不同的互补群。在这三组突变体之间观察到了相当大的遗传相似性。只有三个互补类别是其特定突变体组所特有的，并且在其他两组突变体中未观察到遗传上等效的突变体。源自MHV-A59的突变体组之间有两个重叠的互补群，MHV-JHM组与MHV-A59组之间有四个重叠的互补类别。有两个互补群在所有三组突变体中都有代表性突变体。后一种互补类别之一表现出与基因内互补一致的非互惠互补模式。
