Martin J P, Koehren F, Rannou J J, Kirn A
Laboratoire de Virologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.
Arch Virol. 1988;100(3-4):147-60. doi: 10.1007/BF01487679.
Mouse hepatitis virus 3 (MHV-3) is highly hepatotropic in sensitive mice. Temperature-sensitive mutants (ts mutants) induced by N-methyl-N'-nitrosoguanidine and 5-fluorouracil were isolated. Twelve mutants which were able to induce the formation of syncytia at 33 degrees C but not at the restrictive temperature (39.5 degrees C) were selected for detailed study. No viral RNA synthesis was detected after infection at the restrictive temperature with six of the mutants (RNA-) whereas six others were RNA+, although they displayed RNA synthesis which was generally reduced. No differences have been detected in the size of the genome or the viral-intracellular RNA species found in wild type virus or ts mutant infected cells at permissive temperature. The pattern of virus-induced proteins analyzed after immunoprecipitation by SDS-PAGE was similar in wild type virus and RNA+ mutant infected cells at 39.5 degrees C. Complementation experiments between ts mutants enabled us to distinguish five groups. Three of the groups contained RNA- mutants and two of them RNA+. Plaques made by mutants in one group displayed characteristic features that distinguished them from the wild type.
小鼠肝炎病毒3型（MHV-3）对敏感小鼠具有高度嗜肝性。分离出了由N-甲基-N'-亚硝基胍和5-氟尿嘧啶诱导产生的温度敏感突变体（ts突变体）。选择了12个在33℃能够诱导多核巨细胞形成但在限制温度（39.5℃）不能诱导的突变体进行详细研究。其中6个突变体在限制温度感染后未检测到病毒RNA合成（RNA-），而另外6个是RNA+，尽管它们的RNA合成普遍减少。在允许温度下，野生型病毒或ts突变体感染细胞中发现的基因组大小或病毒细胞内RNA种类没有差异。在39.5℃下，通过SDS-PAGE免疫沉淀分析后，野生型病毒和RNA+突变体感染细胞中病毒诱导蛋白的模式相似。ts突变体之间的互补实验使我们能够区分出五组。其中三组包含RNA-突变体，两组包含RNA+突变体。一组突变体形成的噬斑呈现出与野生型不同的特征。
