Papandreou M J, Fenouillet E
Faculté de Médecine Nord, CNRS, Marseille, France.
Virology. 1998 Feb 1;241(1):163-7. doi: 10.1006/viro.1997.8930.
The influence of HIV Env glycosylation on the conformation of the third variable domain (V3) of Env was studied by both deglycosylation of mature Env and the use of Env produced by recombinant systems in which alpha-glucosidase activity was inhibited by either deoxynojirimycin (DNM) or mutation. Selective deglycosylation affected anti-V3 antibody binding. The immunoreactivity and sensitivity to thrombin cleavage of V3 presented on Env produced in baby hamster kidney cells were changed by DNM treatment. In contrast, Env expressed in alpha-glucosidase I-deficient Chinese hamster ovary cells or in their parental cells treated by DNM fully retained these V3 properties. These results are discussed in relation to the inconsistent data obtained on V3 property changes resulting from Env glycosylation changes.
通过对成熟Env进行去糖基化以及使用重组系统产生的Env（其中α-葡萄糖苷酶活性被脱氧野尻霉素（DNM）或突变抑制），研究了HIV Env糖基化对Env第三个可变结构域（V3）构象的影响。选择性去糖基化影响抗V3抗体结合。DNM处理改变了在幼仓鼠肾细胞中产生的Env上呈现的V3的免疫反应性和对凝血酶切割的敏感性。相反，在α-葡萄糖苷酶I缺陷的中国仓鼠卵巢细胞或经DNM处理的其亲本细胞中表达的Env完全保留了这些V3特性。结合因Env糖基化变化而导致的V3特性变化所获得的不一致数据，对这些结果进行了讨论。
