Stern N J, Myszewski M A, Barnhart H M, Dreesen D W
United States Department of Agriculture-Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30604-5677, USA.
Avian Dis. 1997 Oct-Dec;41(4):899-905.
The purpose of this study was to determine the potential reservoirs for Campylobacter spp. that provide the initial sources involved with broiler chicken colonization during poultry production. We characterized the flagellin A gene (flaA) of the organism by restriction fragment length polymorphism (RFLP) for 59 isolates of the bacterium provided during an epidemiological study. Isolates were obtained from three broiler production houses existing at separate locations. They were cultured and isolated from other (nonbroiler) domestic farm animals, wild birds, rodents, feed, farmers' boots, chicken feathers, and chicken intestinal materials. Eight distinctive flaA types were found in two of the houses. In one house, at least five flaA types (4, 6, 8, 15, and 21) were characterized from the poultry production environment, with three types isolated and identified from the chicken intestinal tract. flaA type 15 was found in flies, on boots, and in chicken intestinal samples. In another house, a distinctive diversity of flaA types existed (4, 7, 43, and 53). At least three flaA types found in samples from chicken intestinal tracts were also found in warm-blooded animals outside of the poultry house (domesticated animals, wild birds, and vermin).
本研究的目的是确定弯曲杆菌属的潜在储存宿主，这些宿主是家禽生产过程中肉鸡定植的初始来源。我们通过限制性片段长度多态性（RFLP）对一项流行病学研究中提供的59株该细菌的鞭毛蛋白A基因（flaA）进行了特征分析。分离株来自位于不同地点的三个肉鸡生产鸡舍。它们是从其他（非肉鸡）家养农场动物、野生鸟类、啮齿动物、饲料、农民的靴子、鸡毛和鸡肠道物质中培养和分离出来的。在其中两个鸡舍中发现了八种不同的flaA类型。在一个鸡舍中，从家禽生产环境中鉴定出至少五种flaA类型（4、6、8、15和21），从鸡肠道中分离并鉴定出三种类型。flaA 15型在苍蝇、靴子和鸡肠道样本中被发现。在另一个鸡舍中，存在独特的flaA类型多样性（4、7、43和53）。在鸡肠道样本中发现的至少三种flaA类型也在家禽舍外的温血动物（家畜、野生鸟类和害虫）中被发现。
