Hänninen M L, Hakkinen M, Rautelin H
Department of Food and Environmental Hygiene, The Haartman Institute, 00231 Helsinki, Finland.
Appl Environ Microbiol. 1999 May;65(5):2272-5. doi: 10.1128/AEM.65.5.2272-2275.1999.
The genomic stability of 12 Campylobacter jejuni strains consisting of two groups of human and chicken isolates was studied by analysis of their PFGE (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks' intestines. The patterns of SmaI, SalI, and SacII digests remained stable after intestinal passage, except for those of two strains. One originally human strain, FB 6371, changed its genotype from II/A (SmaI/SacII) to I/B. Another strain, BTI, originally isolated from a chicken, changed its genotype from I/B to a new genotype. The genomic instability of the strains was further confirmed by SalI digestion and ribotyping of the HaeIII digests. In addition, heat-stable serotype 57 of strain FB 6371 changed to serotype 27 in all isolates with new genotypes but remained unchanged in an isolate with the original genotype. Serotype 27 of strain BTI remained stable. Our study suggests that during intestinal colonization, genomic rearrangement, as demonstrated by changed PFGE and ribopatterns, may occur.
通过分析12株空肠弯曲菌菌株（包括两组人源和鸡源分离株）在新孵出雏鸡肠道内传代后的脉冲场凝胶电泳（PFGE）图谱，研究了它们的基因组稳定性。除两株菌株外，经肠道传代后，SmaI、SalI和SacII酶切图谱保持稳定。一株最初为人源的菌株FB 6371，其基因型从II/A（SmaI/SacII）变为I/B。另一株菌株BTI最初从鸡中分离得到，其基因型从I/B变为一种新的基因型。通过SalI酶切和HaeIII酶切产物的核糖体分型进一步证实了这些菌株的基因组不稳定性。此外，菌株FB 6371的热稳定血清型57在所有具有新基因型的分离株中变为血清型27，但在具有原始基因型的分离株中保持不变。菌株BTI的血清型27保持稳定。我们的研究表明，在肠道定植过程中，可能会发生如PFGE和核糖体图谱改变所显示的基因组重排。
