Carbonell T, Ródenas J, Miret S, Mitjavila M T
Department of Physiology, Faculty of Biology, University of Barcelona, Spain.
Free Radic Res. 1997 Dec;27(6):591-7. doi: 10.3109/10715769709097862.
We investigated the effects of diets with different fatty acid composition upon the oxidative stress of inflammatory leukocytes of rats. After weaning, two groups of rats were fed isoenergetic semipurified diets for five weeks containing 5% of corn oil or menhaden oil. Polymorphonuclear leukocytes from rats fed menhaden oil diet incorporated n-3 polyunsaturated fatty acids into phospholipid membranes at the expense of arachidonic acid. These cells showed diminished superoxide production and, as a consequence, the total antioxidant status in the inflammatory exudate was increased. However, nitric oxide production was not affected by diet. Free malondialdeyde concentration increased in the exudate because of lower mitochondrial activity. These results add new aspects that help clarifying the anti-inflammatory mechanisms of n-3 polyunsaturated fatty acids.
我们研究了不同脂肪酸组成的饮食对大鼠炎性白细胞氧化应激的影响。断奶后，两组大鼠被喂食等能量的半纯化饮食，持续五周，饮食中分别含有5%的玉米油或鲱鱼油。喂食鲱鱼油饮食的大鼠的多形核白细胞将n-3多不饱和脂肪酸纳入磷脂膜，代价是花生四烯酸。这些细胞的超氧化物生成减少，因此，炎性渗出液中的总抗氧化状态增加。然而，一氧化氮的生成不受饮食影响。由于线粒体活性较低，渗出液中的游离丙二醛浓度增加。这些结果为阐明n-3多不饱和脂肪酸的抗炎机制增添了新的内容。
