McNaughton J L, Day E J, Dilworth B C
Poult Sci. 1976 May;55(3):981-6. doi: 10.3382/ps.0550981.
Two broiler chick experiments wer conducted to evaluate four basal diets for iron bioassay suitability. The test basal diets, identified according to their principal ingredients and iron content, were: (1) starch-skim milk - 15 p.p.m., (2) degerminated corn-skin milk - 18 p.p.m. (3) degermianted corn-fish meal-isolated soy - 45 p.p.m., and (4) degermianted corn-fish meal-dehulled soy - 59p.p.m. Significant differences between an iron source with known low availability (ferric oxide) and a highly available iron source (ferrous sulfate) were not detected with the degerminated corn-fish meal-isolated soy or the degerminated corn-fish meal-dehulled soy diets. Likewise, there were no significant differences found between supplemental iron levels, 10 and 20 p.p.m. The corn-skim milk and starch-skim milk diets were both found to be satisfactory for iron bioassays. However, the sample size needed to estimate the population mean was almost twice as great for the starch-skim milk fed groups, than was needed for the corn-skim milk fed groups which indicates the corn-skim milk diet obtained greater sensitivity in testing iron sources and levels. Mortality was excessively high in the starch-skim milk fed group. Ferrous sulfate was superior to ferric oxide as a source of iron.
进行了两项肉鸡雏鸡试验，以评估四种基础日粮对铁生物测定的适用性。根据其主要成分和铁含量确定的试验基础日粮为：（1）淀粉-脱脂乳-15 ppm，（2）脱胚玉米-皮乳-18 ppm，（3）脱胚玉米-鱼粉-分离大豆-45 ppm，以及（4）脱胚玉米-鱼粉-去皮大豆-59 ppm。在脱胚玉米-鱼粉-分离大豆或脱胚玉米-鱼粉-去皮大豆日粮中，未检测到已知低利用率铁源（三氧化二铁）和高利用率铁源（硫酸亚铁）之间的显著差异。同样，在10 ppm和20 ppm的补充铁水平之间也未发现显著差异。玉米-脱脂乳和淀粉-脱脂乳日粮都被认为对铁生物测定是令人满意的。然而，对于喂食淀粉-脱脂乳的组，估计总体均值所需的样本量几乎是喂食玉米-脱脂乳组的两倍，这表明玉米-脱脂乳日粮在测试铁源和水平时具有更高的灵敏度。喂食淀粉-脱脂乳的组死亡率过高。硫酸亚铁作为铁源优于三氧化二铁。
