脂肪酶的立体特异性。脂蛋白脂肪酶对对映体烷基二酰基甘油和二烷基酰基甘油的酶促水解作用。
Stereospecificity of lipases. Enzymatic hydrolysis of enantiomeric alkyldiacyl- and dialkylacylglycerols by lipoprotein lipase.
作者信息
Paltauf F, Wagner E
出版信息
Biochim Biophys Acta. 1976 May 27;431(2):359-62. doi: 10.1016/0005-2760(76)90156-9.
PMID:945746
Abstract
Lipoprotein lipase from dialyzed and lyophilized bovine skim milk hydrolyses specifically the ester in position 1 of triacylglycerols and of enantiomeric alkyldiacylglycerols. No such specificity could be observed when enantiomeric dialkylacylglycerols were used as substrates since hydrolysis in positions 1 and 3 occurred at the same rate. Dialkylacylglycerols are, therefore, unsuitable as model substrates for the assay of the stereospecificity of lipases.
摘要
来自透析并冻干的牛脱脂乳中的脂蛋白脂肪酶特异性水解三酰甘油和对映体烷基二酰甘油1位上的酯。当使用对映体二烷基酰甘油作为底物时，未观察到这种特异性，因为1位和3位的水解速率相同。因此，二烷基酰甘油不适用于作为脂肪酶立体特异性测定的模型底物。
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