Morley N, Kuksis A
Biochim Biophys Acta. 1977 May 25;487(2):332-42. doi: 10.1016/0005-2760(77)90009-1.
Native soybean and rapeseed oils and native and rearranged cod liver and peanut oils were subjected to partial hydrolysis with milk lipoprotein lipase and the fatty acid composition and molecular association in the substrates and lipolysis products were determined. In both native and rearranged oils the lack of significant differences in the fatty acid composition and molecular association between the residual and total triacylglycerols suggested that all triacylglycerols were attacked by the lipoprotein lipase at about the same rate. Any enrichment of a specific fatty acid in the diacyl- or monoacylglycerol products of a native oil generally reflected the preferential association of the fatty acid with the 2-position, and to a lesser extent the sn-3-position of the glycerol molecule and was accompanied by a decreased level of the corresponding free fatty acid product. In general, the products of the rearranged oils closely resembled the original triacylglycerols in the fatty acid composition. It is concluded that lipoprotein lipase does not show any detectable specificity for the unsaturated and polyunsaturated fatty acids with double bonds located at carbons 3 to 19 from the carboxyl end of the fatty acid molecules. These findings are compatible with the possible binding of the substrate to lipoprotein lipase through atoms involved in the acyl ester groups of the triacylglycerol molecules.
将天然大豆油和菜籽油以及天然和重排的鳕鱼肝油和花生油用乳脂蛋白脂肪酶进行部分水解，并测定底物和脂解产物中的脂肪酸组成及分子缔合情况。在天然油和重排油中，残留三酰甘油和总三酰甘油之间的脂肪酸组成及分子缔合均无显著差异，这表明所有三酰甘油被脂蛋白脂肪酶攻击的速率大致相同。天然油的二酰甘油或单酰甘油产物中特定脂肪酸的任何富集通常反映了该脂肪酸优先与甘油分子的2-位缔合，在较小程度上与sn-3-位缔合，同时相应游离脂肪酸产物的水平降低。一般来说，重排油的产物在脂肪酸组成上与原始三酰甘油非常相似。得出的结论是，脂蛋白脂肪酶对脂肪酸分子羧基端碳3至19位带有双键的不饱和及多不饱和脂肪酸没有任何可检测到的特异性。这些发现与底物可能通过三酰甘油分子酰基酯基团中的原子与脂蛋白脂肪酶结合的情况相符。
