Kanno H, Saito K, Ogawa T, Takeda M, Iwasaki A, Kinoshita Y
Department of Urology, Yokohama City University School of Medicine, Japan.
Fertil Steril. 1998 Jan;69(1):127-31. doi: 10.1016/s0015-0282(97)00439-1.
To assess the viability and function of human sperm in electrolyte-free cold preservation.
Prospective comparative study.
Andrology laboratory of our hospital.
PATIENT(S): Ten semen samples obtained from patients attending our infertility clinic.
INTERVENTION(S): Ejaculated sperm were washed using the electrolyte-free Percoll gradient and were then preserved in 0.33 M glucose solution, 0.16 M NaCl solution, 0.16 M KCl solution at 4 degrees C for 4 weeks. As a control, TEST (TES and Tris) yolk buffer (TYB) was added to the ejaculated semen and preserved at 4 degrees C.
MAIN OUTCOME MEASURE(S): Sperm tail morphology, motility, viability (eosin-Y stain), and the concentration of adenosine triphosphate (ATP) were analyzed.
RESULT(S): The number of sperm with normal tail form and the motility of sperm preserved in glucose solution (electrolyte-free cold preservation) were significantly (P < 0.01) higher for 4 weeks than those of sperm preserved in the other three media. The sperm viability in glucose solution was 75.5%, 65.4%, and 51.3%, after 1, 2, and 4 weeks, respectively. The ATP concentration after 1, 2, and 4 weeks remained 64.2%, 53.0%, and 4.3% of the prestorage value, respectively, in the sperm stored in glucose solution.
CONCLUSION(S): The morphology, motility, viability, and ATP concentration of sperm in electrolyte-free cold preservation were substantially better than those in NaCl solution, KCl solution, or TYB for 2 weeks.
评估人精子在无电解质冷保存中的活力和功能。
前瞻性比较研究。
我院男科实验室。
从我院不孕不育门诊患者中获取的10份精液样本。
用无电解质的Percoll梯度洗涤射出的精子，然后将其保存在0.33M葡萄糖溶液、0.16M氯化钠溶液、0.16M氯化钾溶液中，于4℃保存4周。作为对照，向射出的精液中加入TEST（TES和Tris）卵黄缓冲液（TYB）并于4℃保存。
分析精子尾部形态、活力、生存力（伊红-Y染色）和三磷酸腺苷（ATP）浓度。
在葡萄糖溶液（无电解质冷保存）中保存4周的精子，其尾部形态正常的精子数量和活力显著高于在其他三种培养基中保存的精子（P<0.01）。葡萄糖溶液中的精子生存力在1周、2周和4周后分别为75.5%、65.4%和51.3%。在葡萄糖溶液中保存的精子，1周、2周和4周后的ATP浓度分别为储存前值的64.2%、53.0%和4.3%。
在无电解质冷保存中，精子的形态、活力、生存力和ATP浓度在2周内明显优于在氯化钠溶液、氯化钾溶液或TYB中的情况。
