Ruggeri P, Valenti A, Fimiani V
Institute of Biochemistry, Viale Italia 137, 98122 Messina, Italy.
Oncol Rep. 1998 Jan-Feb;5(1):131-4.
LDH isoenzyme shifts are frequently observed in several malignant neoplasm when compared with the corresponding normal tissues. In the present work, we studied LDH isoenzyme behaviour both in Yoshida cells and in founder macrophages, before and after polyamine treatment. The choice of polyamine was prompted in that these polycations also act on the enzymatic and metabolic steps. Results obtained evidenced: i) LDH activity was higher in Yoshida cells than in macrophages; ii) polyamine treatment provoked in tumour cells a very strong increase of LDH activity whereas in macrophages only a slight decrease; iii) Yoshida cells showed the presence of 4 LDH isoenzymes only.
与相应的正常组织相比，乳酸脱氢酶（LDH）同工酶谱在几种恶性肿瘤中经常被观察到。在本研究中，我们研究了多胺处理前后吉田细胞和原始巨噬细胞中LDH同工酶的行为。选择多胺是因为这些聚阳离子也作用于酶促和代谢步骤。获得的结果证明：i）吉田细胞中的LDH活性高于巨噬细胞；ii）多胺处理使肿瘤细胞中的LDH活性大幅增加，而巨噬细胞中仅略有下降；iii）吉田细胞仅显示存在4种LDH同工酶。
