Büttow N C, Miranda Neto M H, Bazotte R B
Department of Morphophysiological Science, Universidade Estadual de Maringá, PR, Brazil.
Arq Gastroenterol. 1997 Jan-Mar;34(1):34-42.
The purpose of this work was to study the morphological and quantitative alterations of the myenteric plexus neurons of the duodenum of rats with acute and chronic streptozotocin-induced diabetes and establish a comparison with non-diabetic animals. Samples of duodenum were destined to histological sections stained by Hematoxilin-Eosin and to membrane preparings stained by the Giemsa and NADH-diaphorasis methods. Small, medium and large neurons were found, with a predominance of medium ones on chronic and acute diabetic animals. It was verified that most of the neurons of diabetic and non-diabetic animals have an eccentrical nucleus and thus this characteristic is not an indicative of degenerative process. It was observed that in diabetes there is a decrease in the number of myenteric neurons. It is argued that this initial decrease is due to the toxic effects of the drug and not to the physiopathology of diabetes itself, and also that the expressive smaller proportion of neurons on the chronically diabetic animals is due to the immediate loss related to streptozotocin and the further consequences of aging during nine weeks of diabetes.
这项工作的目的是研究急性和慢性链脲佐菌素诱导的糖尿病大鼠十二指肠肌间神经丛神经元的形态学和定量变化，并与非糖尿病动物进行比较。十二指肠样本用于苏木精-伊红染色的组织学切片以及吉姆萨染色和NADH黄递酶法染色的膜标本制备。发现了小、中、大神经元，在慢性和急性糖尿病动物中以中型神经元为主。证实糖尿病和非糖尿病动物的大多数神经元都有一个偏心核，因此这一特征并非退行性过程的指标。观察到糖尿病时肌间神经元数量减少。有人认为，这种最初的减少是由于药物的毒性作用，而非糖尿病本身的病理生理学，并且慢性糖尿病动物中神经元比例明显较小是由于与链脲佐菌素相关的直接损失以及糖尿病九周期间衰老的进一步后果。
