Lazar E R, Jolly D T, Tam Y K, Hrazdil J, Tawfik S R, Clanachan A S
Department of Anesthesia, University of Alberta, Edmonton, Canada.
Anesth Analg. 1998 Feb;86(2):422-6. doi: 10.1097/00000539-199802000-00038.
Propofol and thiopental have been used clinically in combination for induction of anesthesia. Studies suggest that this mixture has synergistic activity, recovery characteristics similar to propofol alone, and bactericidal effects on multiple organisms. It may therefore be both clinically useful and cost-effective. In this study, we examined the chemical stability of this mixture. We used high-performance liquid chromatography to quantify the concentration of both propofol and thiopental in a given sample. This technique allows the detection of loss in total drug mass and of the appearance of breakdown products resulting from drug interaction. Ten samples of a 1:1 mixture by volume were prepared and assayed at Time 0 and Days 1, 3, and 7. Half the samples were incubated at 23 degrees C and the rest were stored at 4 degrees C. Other mixtures were assayed before and after filtration at Time 0 and Days 1 and 7 after storage at 23 degrees C. The assay was able to measure accurately the quantity of drug present in the samples. There was no significant decrease in the quantities of either propofol or thiopental in the mixture over the 7-day period. We conclude that the 1:1 volume mixture of propofol and thiopental is chemically stable for 1 wk at room temperature.
A mixture of propofol and thiopental has been used to induce anesthesia. We investigated the chemical stability of this mixture using high-performance liquid chromatography and found it to be stable for at least 24 h.
丙泊酚和硫喷妥钠已在临床上联合用于诱导麻醉。研究表明，这种混合物具有协同活性，恢复特征与单独使用丙泊酚相似，并且对多种微生物有杀菌作用。因此，它可能在临床上有用且具有成本效益。在本研究中，我们检测了这种混合物的化学稳定性。我们使用高效液相色谱法定量给定样品中丙泊酚和硫喷妥钠的浓度。该技术能够检测总药物质量的损失以及药物相互作用产生的分解产物的出现。制备了10个体积比为1:1的混合物样品，并在时间0、第1天、第3天和第7天进行检测。一半样品在23℃下孵育，其余样品保存在4℃。其他混合物在23℃储存后的时间0、第1天和第7天过滤前后进行检测。该检测能够准确测量样品中存在的药物量。在7天期间，混合物中丙泊酚或硫喷妥钠的量均无显著下降。我们得出结论，丙泊酚和硫喷妥钠的1:1体积混合物在室温下化学稳定1周。
丙泊酚和硫喷妥钠的混合物已用于诱导麻醉。我们使用高效液相色谱法研究了这种混合物的化学稳定性，发现它至少在24小时内稳定。
