使用光纤生物传感器快速检测D-二聚体。

Rapid detection of D-dimer using a fiber optic biosensor.

作者信息

Rowe C A, Bolitho J S, Jane A, Bundesen P G, Rylatt D B, Eisenberg P R, Ligler F S

机构信息

Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC 20375-5438, USA.

出版信息

Thromb Haemost. 1998 Jan;79(1):94-8.

PMID:9459331

Abstract

We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.

摘要

我们描述了一种快速灵敏的方法，用于检测和定量D - 二聚体及其他交联纤维蛋白降解产物（XL - FDPs），这些物质在脓毒症和血栓形成疾病患者体内浓度升高。该方法利用固定在倏逝波生物传感器传感区域的夹心荧光免疫测定法。可在11分钟内在校准纤维上的缓冲液和血浆样本中测定D - 二聚体和高分子量XL - FDP的生理浓度。使用酶联免疫吸附测定法（ELISA）和光纤法对脓毒症患者的样本进行检测；光纤测定法测定的浓度与ELISA测定的浓度高度相关（r = 0.918）；批内和批间误差与ELISA相当。鉴于其准确性和快速响应时间，这种光纤生物传感器作为诊断工具具有巨大的开发潜力。