Schmitt J, Keil G M
Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.
J Gen Virol. 1998 Jan;79 ( Pt 1):133-41. doi: 10.1099/0022-1317-79-1-133.
The bovine herpesvirus 1 (BHV-1) strain Schönböken UL8 gene and the 5' flanking region were sequenced. Comparison of the UL8 ORF with the previously reported UL8 ORF of BHV-1 strain Cooper revealed significant differences that were mainly due to three frame-shifted segments. Reanalysis of the Cooper sequence after isolation of the respective segments from genomic DNA by PCR did not confirm the discrepancies; on the contrary, our results indicate a high degree of sequence conservation between the UL8 proteins of different BHV-1 isolates. A monospecific antiserum, raised against a bacterially expressed TrpE-UL8 fusion protein, identified the 80 kDa apparent molecular mass UL8 polypeptide which is localized in the nucleus of infected cells. Analysis of transcripts and time-course studies demonstrated that the UL8 protein is translated from a delayed-early expressed 3.1 kb polyadenylated mRNA which initiates within the UL9 ORF.
对牛疱疹病毒1型（BHV-1）Schönböken株的UL8基因及其5'侧翼区域进行了测序。将UL8开放阅读框与先前报道的BHV-1 Cooper株的UL8开放阅读框进行比较，发现存在显著差异，主要是由于三个移码片段。通过PCR从基因组DNA中分离出各个片段后对Cooper序列进行重新分析，并未证实这些差异；相反，我们的结果表明不同BHV-1分离株的UL8蛋白之间具有高度的序列保守性。用针对细菌表达的TrpE-UL8融合蛋白产生的单特异性抗血清，鉴定出表观分子量为80 kDa的UL8多肽，其定位于受感染细胞的细胞核中。转录本分析和时间进程研究表明，UL8蛋白由一个在UL9开放阅读框内起始的延迟早期表达的3.1 kb多聚腺苷酸化mRNA翻译而来。
