Nerlich A G, Boos N, Wiest I, Aebi M
Pathologisches Institut der Universität, München, Germany.
Virchows Arch. 1998 Jan;432(1):67-76. doi: 10.1007/s004280050136.
We used complete transverse sections through 65 samples of human lumbar intervertebral discs for immunolocalization of the major interstitial collagen types I, II, III, V, VI and IX. The samples were selected from 47 patients ranging in age from 0 (fetuses) to 86 years. The results were compared with the histological findings in disc tissue degeneration and/or reparative alterations as indicated by tear and cleft formation, chondrocyte proliferation, mucous degeneration, granular matrix changes and fibrocartilage fibrillation. We observed a typical pattern for each antibody and each anatomical structure, with, however, remarkable inter- and intraindividual variability, which could be monitored only by use of the complete transverse sections. Accordingly, collagen I was seen in the normal annulus fibrosus and in the degeneratively altered nucleus pulposus, but not within the end-plate, regardless of degenerative changes. Collagens II and IX were found in the normal nucleus pulposus, the inner annulus fibrosus and the end-plate. The collagen II (and IX) staining seemed to be enhanced in areas of minor degenerative lesions, but reduced in advanced lesions and in the degenerated end-plate. Collagens III and VI were significantly increased in areas of minor to advanced degeneration in all anatomical settings, while collagen V showed only minor changes in its staining pattern. In general, histological signs of tissue degeneration coincided with significant quantitative, but also with certain qualitative, changes in the composition of the collagenous disc matrix. These observations indicate the association of degenerative and/or reparative alterations of the intervertebral disc and changes in the collagenous matrix, but document the variability in the extent of the abnormalities observed.
我们使用了65个人类腰椎间盘的完整横切面样本，用于对主要的间质胶原类型I、II、III、V、VI和IX进行免疫定位。样本取自47名年龄从0岁（胎儿）到86岁的患者。将结果与椎间盘组织退变和/或修复性改变的组织学发现进行比较，这些改变表现为撕裂和裂隙形成、软骨细胞增殖、黏液变性、颗粒状基质变化以及纤维软骨纤维化。我们观察到每种抗体和每个解剖结构都有典型的模式，然而，个体间和个体内存在显著差异，只有使用完整的横切面才能监测到这种差异。因此，无论退变情况如何，在正常的纤维环和退变改变的髓核中都能看到胶原I，但在终板内则看不到。胶原II和IX在正常髓核、纤维环内层和终板中被发现。在轻度退变病变区域，胶原II（和IX）染色似乎增强，但在晚期病变和退变的终板中则减弱。在所有解剖结构中，从轻度到重度退变区域，胶原III和VI显著增加，而胶原V的染色模式仅显示出轻微变化。一般来说，组织退变的组织学迹象与椎间盘胶原基质组成的显著定量变化以及某些定性变化相吻合。这些观察结果表明椎间盘退变和/或修复性改变与胶原基质变化之间存在关联，但记录了所观察到的异常程度的变异性。
