异体成纤维细胞通过减少兔骨赘来改善椎间盘退变。
Allogeneic fibroblasts ameliorate intervertebral disc degeneration by reducing osteophytes in rabbits.
作者信息
Chen Chen, Huang Yizhuo, Shi Lei, Zhou Li, Zhou Shenao, Wan Hongjin, Yang Xiao, Zhao Jie
机构信息
Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Jiao Tong University School of Medicine, Shanghai Ninth People's Hospital, Shanghai, China.
出版信息
Front Med (Lausanne). 2024 Nov 1;11:1488727. doi: 10.3389/fmed.2024.1488727. eCollection 2024.
INTRODUCTION
Low back pain (LBP) was commonly induced by intervertebral disc degeneration (IVDD), which is accompanied by the loss of disc height and osteophyte generation. Cell-based therapy is a promising treatment for preventing the degeneration of interverbral disc. In our study, allogeneic fibroblasts are shown to ameliorate intervertebral disc degeneration by reducing osteophytes in rabbits.
METHODS
We established a rabbits-derived fibroblast (Rab-Fib) which could be expanded in vitro and constructed puncture-induced intervertebral disc degeneration rabbit model. Histologic and imaging examinations and analyses were performed after 2 weeks, 3 months, and 12 months.
RESULTS
Our data indicate that stable and reliably-extracted allogeneic fibroblasts can effectively ameliorate intervertebral disc degeneration by reducing osteophytes.
CONCLUSION
Our study provides a basis for advancing the further translation of fibroblasts in intervertebral disc therapy.
引言
下腰痛（LBP）通常由椎间盘退变（IVDD）引起，椎间盘退变伴有椎间盘高度降低和骨赘形成。基于细胞的疗法是预防椎间盘退变的一种有前景的治疗方法。在我们的研究中，同种异体成纤维细胞被证明可通过减少兔子的骨赘来改善椎间盘退变。
方法
我们建立了一种可在体外扩增的兔源成纤维细胞（Rab-Fib），并构建了穿刺诱导的椎间盘退变兔模型。在2周、3个月和12个月后进行组织学和影像学检查及分析。
结果
我们的数据表明，稳定且可靠提取的同种异体成纤维细胞可通过减少骨赘有效改善椎间盘退变。
结论
我们的研究为推进成纤维细胞在椎间盘治疗中的进一步转化应用提供了依据。
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