Schlöricke E, Schmidt H, Schulze H A, Beck R, Guthoff R, Falkenhagen U
Institut für Medizinische Mikrobiologie, Universität Rostock.
Ophthalmologe. 1997 Nov;94(11):785-90. doi: 10.1007/s003470050204.
A major goal in research on intraocular lenses (IOL) is the development of new polymers and modifications to reduce foreign-body reactions after implantation. This effect may be achieved by a reduction in the surface hydrophobicity of the polymers. To illustrate the influence of surface modifications on bacterial adhesiveness, the most often isolated organism in "low-grade" postoperative endophthalmitis, Staphylococcus epidermidis, was used.
For this reason three strains of this species, the type strain ATCC 14990 and two clinical isolates (8687, 6579 I) with different hydrophobic surface properties were studied. IOL, used in the experiments were either made of PMMA or silicone with modified surfaces (unpolished, polished, heparinized). The adhesiveness of H3-thymidin-labeled bacteria was calculated/mm2 of lens surface. Each experiment was performed in triplicate and repeated three times.
The hydrophobic-type strain showed stronger adherence to unpolished PMMA surface (8000 bacteria per mm2) compared to the polished (5200 bacteria/mm2). In contrast, the hydrophilic strain adhered with 2000 bacteria/mm2 to the unpolished and with 4200 bacteria/mm2 to the polished surface. Polishing PMMA lenses diminished the differences between the three strains. However, surface passivation of silicone lenses increased the adhesion rate of the hydrophilic strain up to 9600 bacteria/mm2. Treatment of PMMA lenses with heparin increased the adhesiveness of the hydrophilic strain and reduced the adhesion rate of the hydrophobic type strain to 250 bacteria/mm2.
It was demonstrated that bacterial adherence to IOL also involves hydrophobic interactions. Obviously, however, that adherence reflects a complex of interactions between the two surfaces.
人工晶状体（IOL）研究的一个主要目标是开发新的聚合物并进行改性，以减少植入后的异物反应。这种效果可以通过降低聚合物的表面疏水性来实现。为了说明表面改性对细菌粘附性的影响，使用了在“低度”术后眼内炎中最常分离出的生物体——表皮葡萄球菌。
因此，研究了该物种的三株菌株，即模式菌株ATCC 14990和两株具有不同疏水表面特性的临床分离株（8687、6579 I）。实验中使用的人工晶状体由聚甲基丙烯酸甲酯（PMMA）或具有改性表面（未抛光、抛光、肝素化）的硅酮制成。计算每平方毫米晶状体表面H3-胸苷标记细菌的粘附性。每个实验重复三次，共进行三次。
与抛光表面（每平方毫米5200个细菌）相比，疏水型菌株对未抛光的PMMA表面表现出更强的粘附性（每平方毫米8000个细菌）。相比之下，亲水性菌株在未抛光表面的粘附数为每平方毫米2000个细菌，在抛光表面为每平方毫米4200个细菌。抛光PMMA晶状体减小了三株菌株之间的差异。然而，硅酮晶状体的表面钝化使亲水性菌株的粘附率提高到每平方毫米9600个细菌。用肝素处理PMMA晶状体增加了亲水性菌株的粘附性，并将疏水型菌株的粘附率降低到每平方毫米250个细菌。
结果表明，细菌对人工晶状体的粘附也涉及疏水相互作用。然而，显然这种粘附反映了两个表面之间复杂的相互作用。
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