Schmidt H, Schlöricke E, Fislage R, Schulze H A, Guthoff R
Institute of Medical Microbiology, University of Rostock.
Zentralbl Bakteriol. 1998 Jan;287(1-2):135-45. doi: 10.1016/s0934-8840(98)80159-x.
The development of polymers with different surface properties and surface modifications of intraocular lenses (IOL) should reduce foreign body reactions after implantation by reducing the surface hydrophobicity of the lenses. It was examined how far such surface variations influenced the adhesiveness of bacteria. The most common organism isolated from cases of postoperative endophthalmitis is Staphylococcus epidermidis. For this reason, three strains of this species, the type strain ATCC 14990 and two clinical isolates (8687, 6579 I), with different hydrophobic surfaces, were studied. IOL made of PMMA, silicone, and a copolymer as well as PMMA lenses with modified surfaces (unpolished, polished, silanized, and heparinized) were used. Bacteria were radiolabelled with 3H-thymidine and the adherent bacteria were calculated per mm2 of lens surface. The three strains adhered better to the unpolished surface of silicone than to PMMA. Treatment of PMMA surface by polishing diminished the differences between the strains. An influence of hydrophobic interactions on the adherence of S. epidermidis ATCC 14990 was demonstrated. The adherence of this hydrophobic type strain was clearly reduced by heparinization of the PMMA surface. In contrast, the hydrophilic catheter isolate 6579 I adhered better to modified surfaces. This strain differed clearly in its PFGE pattern from both hydrophobic strains. Hydrophobic interactions play a role in the bacterial adherence to intraocular lenses in vitro and in vivo. Modifications of polymer surfaces, however, can result in rather different effects depending on the bacterial surface composition and properties.
开发具有不同表面特性的聚合物以及对人工晶状体（IOL）进行表面改性，应通过降低晶状体的表面疏水性来减少植入后的异物反应。研究了这种表面变化对细菌粘附性的影响程度。从术后眼内炎病例中分离出的最常见微生物是表皮葡萄球菌。因此，研究了该物种的三株菌株，即模式菌株ATCC 14990和两株临床分离株（8687、6579 I），它们具有不同的疏水表面。使用了由聚甲基丙烯酸甲酯（PMMA）、硅酮和共聚物制成的IOL以及具有改性表面（未抛光、抛光、硅烷化和肝素化）的PMMA晶状体。用³H - 胸腺嘧啶核苷对细菌进行放射性标记，并计算每平方毫米晶状体表面的粘附细菌数。这三株菌株在硅酮的未抛光表面上的粘附性比在PMMA上更好。通过抛光处理PMMA表面减少了菌株之间的差异。证实了疏水相互作用对表皮葡萄球菌ATCC 14990粘附的影响。PMMA表面肝素化明显降低了这种疏水模式菌株的粘附性。相比之下，亲水性导管分离株6579 I在改性表面上的粘附性更好。该菌株的脉冲场凝胶电泳（PFGE）图谱与两种疏水菌株明显不同。疏水相互作用在体外和体内细菌对人工晶状体的粘附中起作用。然而，聚合物表面的改性根据细菌的表面组成和特性可能会产生相当不同的效果。
