Hoque M O, Azuma M, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
J Oral Pathol Med. 1998 Jan;27(1):30-3. doi: 10.1111/j.1600-0714.1998.tb02087.x.
We examined the matrix metalloproteinase (MMP) activity and tumor necrosis factor (TNF)-alpha in luminal fluid of 18 extravasation mucoceles and in saliva from Wharton's duct of five patients by means of gelatin zymography and enzyme immunoassay, respectively. The luminal fluid showed a high level of MMP activity compared with the saliva. Quantitative determination by enzyme immunoassay revealed that the luminal fluid contained higher levels of TNF-alpha than the saliva. In addition, the amount of TNF-alpha in luminal fluid exhibited a direct correlation with MMP activity estimated by densitometric analysis of gelatin zymograms. Since TNF-alpha stimulates the production of MMPs from cells such as fibroblasts, these results suggest that TNF-alpha is one of the causal molecules that enhance the accumulation of proteolytic enzymes in luminal fluid of mucoceles.
我们分别通过明胶酶谱法和酶免疫测定法，检测了18例黏液外渗性黏液囊肿管腔内液体中的基质金属蛋白酶（MMP）活性以及5例患者颌下腺导管唾液中的肿瘤坏死因子（TNF）-α。与唾液相比，管腔内液体显示出高水平的MMP活性。酶免疫测定法定量测定显示，管腔内液体中TNF-α的水平高于唾液。此外，通过对明胶酶谱进行光密度分析估计，管腔内液体中TNF-α的量与MMP活性呈直接相关。由于TNF-α可刺激成纤维细胞等细胞产生MMP，这些结果表明TNF-α是增强黏液囊肿管腔内液体中蛋白水解酶积累的致病分子之一。
