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对在巴西收集并点样于滤纸上的结核分枝杆菌复合群培养分离株进行DNA指纹分析。

DNA fingerprinting of Mycobacterium tuberculosis complex culture isolates collected in Brazil and spotted onto filter paper.

作者信息

Burger M, Raskin S, Brockelt S R, Amthor B, Geiss H K, Haas W H

机构信息

GENETIKA, Laboratory of Genetics, Curitiba, Brazil.

出版信息

J Clin Microbiol. 1998 Feb;36(2):573-6. doi: 10.1128/JCM.36.2.573-576.1998.

DOI:10.1128/JCM.36.2.573-576.1998
PMID:9466780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104581/
Abstract

The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens analyzed by an amplification-based typing method provide a new resource for epidemiological studies of infectious diseases.

摘要

通过对77名巴西患者的结核分枝杆菌分离株进行混合连接子DNA指纹分析,证明了滤纸对保存细菌细胞的有效性。点样于滤纸上的样本与传统培养材料的DNA指纹相同。因此,通过基于扩增的分型方法分析的滤纸标本为传染病的流行病学研究提供了一种新资源。

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本文引用的文献

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Interpretation of restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates from a state with a large rural population.对来自一个农村人口众多的邦的结核分枝杆菌分离株进行限制性片段长度多态性分析的解读。
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Epidemiology of tuberculosis by DNA fingerprinting.
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Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.通过DNA指纹图谱技术进行结核分枝杆菌菌株鉴定:标准化方法建议
J Clin Microbiol. 1993 Feb;31(2):406-9. doi: 10.1128/jcm.31.2.406-409.1993.
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Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex.混合连接酶聚合酶链反应:一种用于快速鉴定结核分枝杆菌复合群分离株的新方法。
J Clin Microbiol. 1993 May;31(5):1293-8. doi: 10.1128/jcm.31.5.1293-1298.1993.
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Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis.用IS6110产生的结核分枝杆菌菌株DNA指纹图谱的稳定性。
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In situ PCR amplification of Guthrie card DNA to detect cystic fibrosis mutations.用于检测囊性纤维化突变的干血斑DNA原位PCR扩增。
Clin Chem. 1995 Mar;41(3):477-9.
10
Rendering of mycobacteria safe for molecular diagnostic studies and development of a lysis method for strand displacement amplification and PCR.使分枝杆菌对分子诊断研究安全,并开发用于链置换扩增和聚合酶链反应的裂解方法。
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