Pretorius A, Oelofsen M J, Smith M S, van der Ryst E
Department of Medical Microbiology, Faculty of Medicine, University of the Free State, Bloemfontein, South Africa.
Onderstepoort J Vet Res. 1997 Sep;64(3):201-3.
Infectious diseases of wild animals are of increasing importance, both from an economic viewpoint and because several of these diseases are pathogenic to man. However, serosurveys to determine the circulation of infectious organisms in wildlife are complicated by the fact that antibodies to species-specific immunoglobulins are not available for use in serological assays such as enzyme-linked immunosorbent assays (ELISAs) or immunofluorescence assays. To determine the binding potential of four commercially available antibody conjugates with the sera of wild animals, sera from 27 species of small terrestrial mammals were allowed to react with alkaline phosphatase-labelled protein A, anti-rabbit IgG, anti-mouse IgG and anti-human IgG by by the use of an ELISA. It was found that sera from some species of the order Lagomorpha bound optimally to anti-rabbit IgG, while anti-mouse IgG could be used for most species of Rodentia. For all Carnivora, Insectivora, Macroscelidea, Hyracoidea and other Rodentia, staphylococcal protein A demonstrated optimal binding. None of the sera that was tested bound to anti-human IgG. These results demonstrate that commercial conjugates can be used in serological assays in which wild animal sera are used, and should be useful for future serosurveys to determine the circulation of infectious agents in small terrestrial mammals.
野生动物的传染病正变得越来越重要，这不仅从经济角度来看如此，还因为其中一些疾病对人类具有致病性。然而，由于缺乏用于酶联免疫吸附测定（ELISA）或免疫荧光测定等血清学检测的针对物种特异性免疫球蛋白的抗体，确定野生动物中感染性生物体传播情况的血清学调查变得复杂。为了确定四种市售抗体偶联物与野生动物血清的结合潜力，通过ELISA让27种小型陆生哺乳动物的血清与碱性磷酸酶标记的蛋白A、抗兔IgG、抗小鼠IgG和抗人IgG反应。结果发现，兔形目某些物种的血清与抗兔IgG结合最佳，而抗小鼠IgG可用于大多数啮齿目物种。对于所有食肉目、食虫目、象鼩目、蹄兔目和其他啮齿目动物，葡萄球菌蛋白A显示出最佳结合。所检测的血清均未与抗人IgG结合。这些结果表明，市售偶联物可用于使用野生动物血清的血清学检测，并且对未来确定小型陆生哺乳动物中感染因子传播情况的血清学调查应该是有用的。
