Park Ok-Jin, Jeong Moon-Hee, Lee Eun-Hye, Cho Mi-Ran, Hwang Jaehong, Cho Seungryong, Yun Cheol-Heui, Han Seung Hyun, Kim Sun-Young
Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Korea.
Department of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea.
Microorganisms. 2020 Aug 2;8(8):1174. doi: 10.3390/microorganisms8081174.
Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level, , and were dominant genera in the pulp chamber. The most abundant genera in the root apex were , and . The species increased in the pulp chamber, whereas the species and were highly observed in the root apex as the disease progressed. The experimental rat model of apical periodontitis demonstrated a relationship between the microbiota and the apical periodontitis progression.
由牙髓中的微生物感染引起的根尖周炎的特征是炎症、牙髓和根尖周组织的破坏以及牙槽骨吸收。我们使用基于焦磷酸测序的方法结合大鼠根尖周炎模型中的放射学和组织病理学变化，分析了微生物群的时间变化。在为期三周的观察期间，牙髓和根尖周区域显示出根尖周炎的典型进展。共鉴定出27个门、645个属和1276个物种。根尖的细菌物种多样性低于髓腔。变形菌门、厚壁菌门、拟杆菌门和放线菌门是髓腔和根尖中的优势菌门。值得注意的是，根据疾病进展，根尖中的细菌群落呈现出变化趋势。在属水平上， 、 和 是髓腔中的优势属。根尖中最丰富的属是 、 和 。随着疾病进展， 物种在髓腔中增加，而 物种和 在根尖中高度可见。根尖周炎的实验大鼠模型证明了微生物群与根尖周炎进展之间的关系。
