Peschle C, Marone G, Genovese A, Magli C, Condorelli M
Am J Physiol. 1976 Mar;230(3):845-8. doi: 10.1152/ajplegacy.1976.230.3.845.
Erythropoietin (Ep) levels were evaluated in serum of neonate, weanling, or adult rats subjected to 1) sham operation, nephrectomy, and/or subtotal hepatectomy and 2) a standard bout of hypoxia (0.45 atm air/6 h, starting 1 h after the operation). Ep activity was quantitated by means of strictly controlled assays in exhypoxic polycythemic mice. The sum of Ep titers in the serum of nephrectomized or hepatectomized rats was compared to Ep levels in sham-operated animals of corresponding age levels, with the exception of 1-wk-old rats: it is relevance that no significant difference is apparent between these Ep production curves. Thus, evidence is presented indicating for the first time that Ep derives from two functionally distinct and additive sources, i.e., the kidney and the liver. Liver Ep, although prevalent in neonatal animals, is obscured in the weanling adult rat by both gradual initiation of massive renal Ep production and progressive decrease of hepatic Ep activity.
在接受以下处理的新生、断奶或成年大鼠血清中评估促红细胞生成素（Ep）水平：1）假手术、肾切除术和/或肝部分切除术；2）一次标准的低氧暴露（0.45个大气压空气/6小时，术后1小时开始）。通过在低氧性红细胞增多症小鼠中进行严格控制的测定来定量Ep活性。将肾切除或肝切除大鼠血清中的Ep滴度总和与相应年龄水平的假手术动物的Ep水平进行比较，但1周龄大鼠除外：这些Ep产生曲线之间没有明显差异，这具有相关性。因此，首次有证据表明Ep来自两个功能不同且具有累加作用的来源，即肾脏和肝脏。肝脏Ep虽然在新生动物中普遍存在，但在断奶后的成年大鼠中，由于大量肾脏Ep产生的逐渐启动和肝脏Ep活性的逐渐降低而被掩盖。
