Shimokawa I, Higami Y, Horiuchi S, Iwasaki M, Ikeda T
Department of Pathology, Nagasaki University School of Medicine, Japan.
J Gerontol A Biol Sci Med Sci. 1998 Jan;53(1):B49-51. doi: 10.1093/gerona/53a.1.b49.
The present study examined the presence of advanced glycosylation end products (AGEs) in lipofuscin present in the brain and adrenal gland of aging rats by immunohistochemistry using antibodies raised against AGEs. Lipofuscin identified as yellow to brown granules emitting bright yellow to orange autofluorescence with ultraviolet light were detected in cortical neurons, cerebellar Purkinje cells, and adrenal cells in the inner part of the zona reticularis. However, none of the antibodies visualized lipofuscin in these areas. The outer part of the zona reticularis contained yellow granules emitting a faint orange autofluorescence. These granules were immunostained by an antibody that reacted with AGEs structures unrelated to the carboxymethyllysine moiety. Newly formed adrenal cortical cells are thought to migrate from the outer layer to the inner layer of the zona reticularis. Therefore, our results suggest that glycosylation-related processes are involved in lipofuscinogenesis, at least in its early stage, in the adrenal zona reticularis.
本研究通过使用针对晚期糖基化终产物（AGEs）产生的抗体进行免疫组织化学，检测了衰老大鼠脑和肾上腺中脂褐素内AGEs的存在情况。在皮质神经元、小脑浦肯野细胞以及网状带内部的肾上腺细胞中，检测到脂褐素呈现为黄色至棕色颗粒，在紫外线下发出亮黄色至橙色的自发荧光。然而，这些区域中没有一种抗体能使脂褐素显色。网状带外部含有发出微弱橙色自发荧光的黄色颗粒。这些颗粒被一种与与羧甲基赖氨酸部分无关的AGEs结构反应的抗体免疫染色。新形成的肾上腺皮质细胞被认为是从网状带外层迁移至内层。因此，我们的结果表明，糖基化相关过程至少在肾上腺网状带脂褐素形成的早期阶段参与其中。
