Department of Surgery, University of British Columbia, Vancouver, BC, Canada,
Cytotechnology. 2015 Mar;67(2):379-86. doi: 10.1007/s10616-014-9692-5. Epub 2014 Jan 19.
Cell viability and cell migration capacities are critical parameters for cell culture-related studies. It is essential to monitor the dynamic changes of cell properties under various co-culture conditions to our better understanding of their behaviours and characteristics. The real time cell analyzer (RTCA, xCELLigence, Roche) is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration and cytotoxicity. The practicality of this system has been proven in our recent cancer studies. In the present method, we intend to use co-cultures of pancreatic cancer cells (HP62) and mesenchymal stem cells to describe in detail, the procedures and benefits of RTCA.
细胞活力和细胞迁移能力是细胞培养相关研究的关键参数。在各种共培养条件下监测细胞特性的动态变化对于我们更好地了解它们的行为和特征至关重要。实时细胞分析（RTCA，xCELLigence，罗氏）是一种基于阻抗的技术，可用于无标记和实时监测细胞特性，如细胞黏附、增殖、迁移和细胞毒性。该系统的实用性已在我们最近的癌症研究中得到证实。在本方法中，我们拟采用胰腺癌细胞（HP62）和间充质干细胞共培养来详细描述 RTCA 的步骤和优势。
