Gusinskaia V V, Artiukhov V G, Sergeev K P
Voronezh State University, Russia.
Radiats Biol Radioecol. 1997 Nov-Dec;37(6):926-31.
The structural transformations of C1q-protein induced by the UV-light at doses 151 and 2265 J/m2 has been determined by means of acid-basic titration, IR- ans UV-spectrophotometry. These transformations are stipulated by unfolding of its molecules without change of the secondary structure types ratio and accompanies by exposition on aromatic amino acid residues as well as glutamine and asparagine acids, lysine, hydroxylysine and cysteine on the globule surface. The correlation between structural photomodifications and processes of functional activation of C1q has been established.
通过酸碱滴定、红外和紫外分光光度法测定了剂量为151和2265 J/m²的紫外线诱导的C1q蛋白的结构转变。这些转变是由其分子的展开引起的，二级结构类型比例不变，同时伴随着球状表面上芳香族氨基酸残基以及谷氨酰胺、天冬酰胺酸、赖氨酸、羟赖氨酸和半胱氨酸的暴露。已经建立了结构光修饰与C1q功能激活过程之间的相关性。
