Patiño B, Posada M L, González-Jaén M T, Vázquez C
Departamento de Microbiología III, Facultad de Biologia, Universidad Complutense de Madrid, Spain.
Microbios. 1997;91(366):47-54.
The production of polygalacturonases from Fusarium oxysporum f. sp. radicis lycopersici (FORL) occurred sequentially, and endo- and exo-polygalacturonases (PG) predominated at different times during growth. At first FORL produced a PG which did not release monomers from pectins and was unable to hydrolyse dimers and trimers. Later a complex was produced in which exo-PG seemed to be the predominant activity; the monomer was released rapidly and this activity was able to degrade dimers and trimers.
尖孢镰刀菌番茄根腐专化型(FORL)产生聚半乳糖醛酸酶的过程是循序渐进的,在生长过程中的不同时期,内切和外切聚半乳糖醛酸酶(PG)占主导地位。起初,FORL产生一种不能从果胶中释放单体、也无法水解二聚体和三聚体的PG。后来产生了一种复合物,其中外切PG似乎是主要活性成分;单体迅速释放,这种活性能够降解二聚体和三聚体。