Sasaki Kazunori, Ito Yumi, Hamada Yuki, Dowaki Ayano, Jogaiah Sudisha, Ito Shin-Ichi
Department of Biological and Environmental Sciences, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.
Research Center for Thermotolerant Microbial Resources (RCTMR), Yamaguchi University, Yamaguchi 753-8515, Japan.
Pathogens. 2022 Nov 28;11(12):1433. doi: 10.3390/pathogens11121433.
f. sp. (Forl) causes crown and root rot disease in tomato, effecting severe economic losses. However, research on the pathogenicity genes and infection strategy of Forl is limited compared to that on f. sp. (Fol). In this study, we characterized gene in Forl as a homolog of required for pathogenicity in rice blast pathogen-. Gene expression analysis revealed that expressionin Forl is higher than that in Fol. -knockout mutant of Forl had significantly reduced root rot symptoms compared to the wild-type strain, and full pathogenicity was restored by complementation. By contrast, mutant of Fol presented the same symptoms as the wild type, suggesting that of Forl, but not of Fol, was essential for full virulence in tomato plants. Morphological differences between the Forl and in the roots were observed by fluorescent labeling using WGA-FITC. Chlamydospores of the mutant of Forlcontinuously increased during infection and were three times higher than that of the wild type at 21 days post-inoculation. These observations suggest that of Forl is required for virulence to tomato plants by involving the normal development and germination of chlamydospores.
番茄枯萎病菌（Fusarium oxysporum f. sp. (Forl)）会引发番茄的冠腐病和根腐病，造成严重的经济损失。然而，与尖孢镰刀菌（Fusarium oxysporum f. sp. (Fol)）相比，关于Forl致病基因和侵染策略的研究较为有限。在本研究中，我们将Forl中的一个基因鉴定为稻瘟病菌致病所需基因的同源物。基因表达分析表明，Forl中该基因的表达高于Fol中的表达。Forl的该基因敲除突变体与野生型菌株相比，根腐症状明显减轻，通过互补作用可恢复完全致病性。相比之下，Fol的该基因突变体表现出与野生型相同的症状，这表明Forl的该基因而非Fol的该基因对于番茄植株的完全毒力至关重要。通过使用WGA-FITC进行荧光标记观察到了Forl和Fol在根部的形态差异。Forl的该基因突变体的厚垣孢子在感染过程中持续增加，接种后21天时比野生型高3倍。这些观察结果表明，Forl的该基因通过参与厚垣孢子的正常发育和萌发，对番茄植株的毒力是必需的。
