非切片生物材料的电子显微镜放射自显影术。

EM autoradiography of non-sectioned biological material.

作者信息

Haworth R A, Chapman J A

出版信息

J Microsc. 1976 Mar;106(2):125-30. doi: 10.1111/j.1365-2818.1976.tb02395.x.

DOI:10.1111/j.1365-2818.1976.tb02395.x

Abstract

Methods of EM autoradiography suitable for the study of non-sectioned biological material deposited on a support film from suspension are described. The methods, which include modified procedures for specimen support and emulsion preparation, may also have advantages for the study of any specimen where physical separation of specimen and emulsion is desirable. In a study of the growth of 125I-labelled collagen fibrils reconstituted from solution in vitro, these procedures have allowed the production of EM autoradiographs which show a high resolution and are free from stress artefacts and chemography.

摘要

描述了适用于研究从悬浮液沉积在支撑膜上的非切片生物材料的电子显微镜放射自显影方法。这些方法包括用于标本支撑和乳剂制备的改进程序，对于任何需要将标本与乳剂物理分离的标本研究也可能具有优势。在一项关于从体外溶液中重构的125I标记胶原纤维生长的研究中，这些程序使得能够制作出具有高分辨率、无应力伪影和化学成像的电子显微镜放射自显影片。