与HIV感染患者的外周血相比，其肠道黏膜中的HIV-1 p24增加，但前病毒载量未增加。

HIV-1 p24 but not proviral load is increased in the intestinal mucosa compared with the peripheral blood in HIV-infected patients.

作者信息

Fackler O T, Schäfer M, Schmidt W, Zippel T, Heise W, Schneider T, Zeitz M, Riecken E O, Mueller-Lantzsch N, Ullrich R

机构信息

Department of Virology, University of the Saarland, Homburg/Saar, Germany.

出版信息

AIDS. 1998 Jan 22;12(2):139-46. doi: 10.1097/00002030-199802000-00003.

DOI:10.1097/00002030-199802000-00003

PMID:9468362

Abstract

OBJECTIVE

To investigate differences in viral and proviral load between the peripheral blood and the intestinal mucosal immune system in HIV-infected patients.

DESIGN

HIV-1 p24 and HIV DNA content were compared in blood samples and intestinal biopsies from HIV-infected patients.

METHODS

Intestinal biopsies and peripheral blood were simultaneously obtained from 27 HIV-infected patients undergoing diagnostic endoscopy. The p24 concentrations were measured in serum and homogenized intestinal biopsies by enzyme-linked immunosorbent assay after acid-dissociation of immune complexes. Proviral load was determined in blood and intestinal biopsies by a quantitative competitive polymerase chain reaction amplifying the HIV-1 nef gene from genomic DNA.

RESULTS

No significant differences were found in proviral load comparing HIV copies per 1.5 x 10(5) cell equivalents in blood [2650 (600-44000)] and intestinal biopsies [4200 (1325-19 625)]. Paired analysis revealed a strong positive correlation between serum and mucosal proviral load. In contrast, HIV core protein p24 was detected in intestinal biopsies from 18 patients in much higher concentrations than in serum [858 (262-4111) pg/g versus 34 (9-242) pg/g; P < 0.005]. The p24 concentrations in serum and intestinal biopsies did not correlate and no significant correlation was observed in serum or intestinal biopsies between proviral load and p24 concentrations. No clear correlations were observed between clinical parameters and HIV DNA or HIV p24 levels in blood or biopsies.

CONCLUSIONS

Our findings demonstrate a homogenous distribution of HIV proviral load in the peripheral blood and the intestinal mucosal immune system. The high viral antigen load in the intestine therefore indicates that mucosal HIV production is upregulated at the transcriptional and/or translational level. The intestinal mucosa is a major reservoir for HIV in HIV-infected patients.

摘要

目的

研究HIV感染患者外周血与肠道黏膜免疫系统之间病毒载量和前病毒载量的差异。

设计

比较HIV感染患者血液样本和肠道活检组织中的HIV-1 p24和HIV DNA含量。

方法

从27例接受诊断性内镜检查的HIV感染患者中同时获取肠道活检组织和外周血。免疫复合物经酸解离后，通过酶联免疫吸附测定法测量血清和匀浆后的肠道活检组织中的p24浓度。通过定量竞争性聚合酶链反应从基因组DNA中扩增HIV-1 nef基因，测定血液和肠道活检组织中的前病毒载量。

结果

比较每1.5×10⁵细胞当量血液中的HIV拷贝数[2650（600 - 44000）]和肠道活检组织中的HIV拷贝数[4200（1325 - 19625）]，前病毒载量未发现显著差异。配对分析显示血清和黏膜前病毒载量之间存在强正相关。相比之下，在18例患者的肠道活检组织中检测到的HIV核心蛋白p24浓度远高于血清中的浓度[858（262 - 4111）pg/g对34（9 - 242）pg/g；P < 0.005]。血清和肠道活检组织中的p24浓度不相关，在前病毒载量与p24浓度之间，血清或肠道活检组织中均未观察到显著相关性。血液或活检组织中的临床参数与HIV DNA或HIV p24水平之间未观察到明显相关性。