Lahaye M, Ray B
Laboratoire de Biochimie et Technologie des Glucides, INRA, Nantes, France.
Carbohydr Res. 1996 Mar 22;283:161-73. doi: 10.1016/0008-6215(95)00407-6.
In order to obtain information on sugar sequences in the water soluble polysaccharides from Ulva "rigida", oligosaccharides were purified by anion exchange and gel permeation chromatography from the partial acid hydrolysate of the native or desulfated ulvan. The chemical structure and sugar sequence of these oligomers were determined by 1H and 13C NMR spectroscopy to be alpha-L-rhamnosyl (1-->4) D-xylose, beta-D-glucuronosyluronic acid (1-->2)-alpha-L-rhamnosyl (1-->4) D-xylose, beta-D-glucuronosyluronic acid (1-->4) L-rhamnose 3 sulfate, beta-D-glucuronosyluronic acid (1-->4) [beta-D-glucuronosyluronic acid (1-->2)] L-rhamnose and beta-D-glucuronosyluronic acid (1-->4) [beta-D-glucuronosyluronic acid (1-->2)] alpha-L-rhamnosyl (1-->4) D-xylose. The sugar linkages and particularly the branching and the sulfate position were in total agreement with previous chemical results.
为了获取石莼中水溶性多糖的糖序列信息，从天然或脱硫酸石莼聚糖的部分酸水解产物中，通过阴离子交换和凝胶渗透色谱法纯化寡糖。通过1H和13C NMR光谱确定这些寡聚物的化学结构和糖序列为α-L-鼠李糖基（1→4）D-木糖、β-D-葡萄糖醛酸糖醛酸（1→2）-α-L-鼠李糖基（1→4）D-木糖、β-D-葡萄糖醛酸糖醛酸（1→4）L-鼠李糖3-硫酸盐、β-D-葡萄糖醛酸糖醛酸（1→4）[β-D-葡萄糖醛酸糖醛酸（1→2）]L-鼠李糖和β-D-葡萄糖醛酸糖醛酸（1→4）[β-D-葡萄糖醛酸糖醛酸（1→2）]α-L-鼠李糖基（1→4）D-木糖。糖键，特别是分支和硫酸根位置与先前的化学结果完全一致。
