热休克和抗生素对质体RNA编辑的位点选择性抑制：质体翻译在RNA编辑中的作用
Site-selective inhibition of plastid RNA editing by heat shock and antibiotics: a role for plastid translation in RNA editing.
作者信息
Karcher D, Bock R
机构信息
Institut für Biologie III, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.
出版信息
Nucleic Acids Res. 1998 Mar 1;26(5):1185-90. doi: 10.1093/nar/26.5.1185.
Abstract
RNA editing in higher plant plastids changes single cytidine residues to uridine through an unknown mechanism. In order to investigate the relation of editing to physiological processes and to other steps in plastid gene expression, we have tested the sensitivity of chloroplast RNA editing to heat shock and antibiotics. We show that heat shock conditions as well as treatment of plants with prokaryotic translational inhibitors can inhibit plastid RNA editing. Surprisingly, this inhibitory effect is confined to a limited number of plastid editing sites suggesting that some site-specific factor(s) but none of the general components of the plastid RNA editing machinery are compromised. Contrary to previous expectations, our results provide evidence for a role of plastid translation in RNA editing.
摘要
高等植物质体中的RNA编辑通过一种未知机制将单个胞嘧啶残基转变为尿嘧啶。为了研究编辑与生理过程以及质体基因表达中其他步骤的关系，我们测试了叶绿体RNA编辑对热休克和抗生素的敏感性。我们发现热休克条件以及用原核翻译抑制剂处理植物均可抑制质体RNA编辑。令人惊讶的是，这种抑制作用仅限于有限数量的质体编辑位点，这表明某些位点特异性因子受到影响，但质体RNA编辑机制的一般组分未受影响。与先前的预期相反，我们的结果为质体翻译在RNA编辑中的作用提供了证据。
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