Pacheco M, Santos M A
Department of Biology, University of Aveiro, Portugal.
Ecotoxicol Environ Saf. 1997 Dec;38(3):252-9. doi: 10.1006/eesa.1997.1585.
The purpose of this research is the study of total ethoxyresorufin O-deethylase (EROD) activity and erythrocytic nuclear abnormalities (NA) induction by polycyclic aromatic hydrocarbons (PAHs) and resin acids (RAs) on the juvenile eel Anguilla anguilla L. Juvenile eels were exposed, during 3 days, to the same molar concentration range (0, 0.1, 0.3, 0.9, and 2.7 microM) of beta-naphthoflavone (BNF) and benzo[a]pyrene (BP)--PAHs--and abietic acid (AA) and dehydroabietic acid (DHAA)--RAs--and one alkylating agent, such as cyclophosphamide (CP). Results revealed that both PAHs are able to significantly increase total EROD activity at 0.9 and 2.7 microns, and both RAs at 0.3 microM, whereas NA frequency was significantly increased by BP at 0.9 microM and DHAA at 2.7 microM. BNF was found to be the most potent EROD inducer, and BP exhibited the highest mutagenic potential. An additional experiment was carried out in order to study the BNF total EROD and NA induction over a period of 3, 6, and 9 days. An increased length of exposure, beyond 3 days, does not proportionally increase total EROD activity. However, 6 and 9 days BNF exposure significantly increases NA, reaching the (NA) levels previously determined for 3 days 0.9 microM BP exposure.
本研究的目的是研究多环芳烃（PAHs）和树脂酸（RAs）对欧洲鳗鲡幼鱼的总乙氧基异吩唑酮-O-脱乙基酶（EROD）活性和红细胞核异常（NA）的诱导作用。将欧洲鳗鲡幼鱼暴露于相同摩尔浓度范围（0、0.1、0.3、0.9和2.7微摩尔）的β-萘黄酮（BNF）和苯并[a]芘（BP）——PAHs——以及枞酸（AA）和脱氢枞酸（DHAA）——RAs——和一种烷基化剂，如环磷酰胺（CP）中，持续3天。结果显示，两种PAHs在0.9和2.7微摩尔时均能显著提高总EROD活性，两种RAs在0.3微摩尔时能提高该活性，而NA频率在0.9微摩尔的BP和2.7微摩尔的DHAA作用下显著增加。发现BNF是最有效的EROD诱导剂，而BP具有最高的诱变潜力。为了研究BNF在3、6和9天内对总EROD和NA的诱导作用，还进行了一项额外的实验。暴露时间超过3天，总EROD活性不会成比例增加。然而，暴露于BNF 6天和9天会显著增加NA，达到之前3天暴露于0.9微摩尔BP时所确定的（NA）水平。
