Oh K B, Nishiyama T, Sakai E, Matsuoka H, Kurata H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184, Japan.
J Biotechnol. 1997 Dec 3;58(3):197-204. doi: 10.1016/s0168-1656(97)00147-8.
Hyphae of a variety of mycelial fungi were shown to reorient their direction of growth to grow upstream in a continuous flowing current of liquid medium. Among those tested fungi, Aspergillus niger hyphae was most polarized towards the upstream. Hyphae of A. niger which had been attached to poly-L-lysine coated plates on the base of a continuous-culture flow cell were exposed to a current of medium of flow rates in the range of 0-6400 microns s-1 for 1-4 h. Hyphal tips quickly reoriented towards the upstream of the flowing medium and became aligned parallel to the flow axis as they grew larger. At flow rates above 1600 microns s-1, however, the percentage of polarization of hyphae reached a constant level. With other fungi, germ tube emergence was not polarized but hyphae and branches grew towards the upstream. There was no detectable difference in the pH and oxygen concentration of the flowing medium between inlet and outlet of the flow cell. This result suggests that hyphae of mycelial fungi sense transient flow of liquid medium over their surface and reorient their direction to grow upstream.
多种丝状真菌的菌丝被证明能够重新定向其生长方向，以便在连续流动的液体培养基中向上游生长。在测试的真菌中，黑曲霉的菌丝向上游的极化程度最高。将附着在连续培养流动池底部聚-L-赖氨酸包被平板上的黑曲霉菌丝暴露于流速范围为0 - 6400微米/秒的培养基流中1 - 4小时。菌丝尖端迅速向上游流动的培养基重新定向，并随着它们的生长而与流动轴平行排列。然而，在流速高于1600微米/秒时，菌丝的极化百分比达到一个恒定水平。对于其他真菌，芽管的出现没有极化，但菌丝和分支向上游生长。流动池的入口和出口之间流动培养基的pH值和氧气浓度没有可检测到的差异。这一结果表明，丝状真菌的菌丝能够感知其表面液体培养基的瞬时流动，并重新定向其生长方向向上游生长。
