一种能够调节白色念珠菌形态转变的自调节物质的纯化与特性分析。
Purification and characterization of an autoregulatory substance capable of regulating the morphological transition in Candida albicans.
作者信息
Oh K B, Miyazawa H, Naito T, Matsuoka H
机构信息
Natural Products Research Institute, Seoul National University, 28, Yungun, Chongro, Seoul 110-460,
出版信息
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4664-8. doi: 10.1073/pnas.071404698. Epub 2001 Mar 27.
Abstract
The yeast Candida albicans has a distinguishing feature, dimorphism, which is the ability to switch between two morphological forms: a budding yeast form and a multicellular invasive filamentous form. This ability has been postulated to contribute to the virulence of this organism. Studies on the morphological transition from a filamentous to a budding yeast form in C. albicans have shown that this organism excretes an autoregulatory substance into the culture medium. This substance was extracted and purified by normal-phase and reversed-phase HPLC. The autoregulatory substance was structurally identified as 3,7,11-trimethyl-2,6,10-dodecatrienoate (farnesoic acid) by NMR and mass spectrometry. Growth experiments suggest that this substance does not inhibit yeast cell growth but inhibits filamentous growth. These findings have implications for developmental signaling by the fungus and might have medicinal value in the development of antifungal therapies.
摘要
白色念珠菌具有一个显著特征，即二态性，也就是能够在两种形态之间转换：出芽酵母形态和多细胞侵袭性丝状形态。据推测，这种能力有助于该生物体的毒力。对白色念珠菌从丝状形态向出芽酵母形态的形态转变的研究表明，该生物体向培养基中分泌一种自调节物质。这种物质通过正相和反相高效液相色谱法进行提取和纯化。通过核磁共振和质谱分析，将该自调节物质的结构鉴定为3,7,11-三甲基-2,6,10-十二碳三烯酸酯（法尼酸）。生长实验表明，这种物质并不抑制酵母细胞生长，但会抑制丝状生长。这些发现对真菌的发育信号传导具有启示意义，并且可能在抗真菌治疗的开发中具有药用价值。
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本文引用的文献
1
Differential activation of a Candida albicans virulence gene family during infection.白色念珠菌毒力基因家族在感染过程中的差异激活。
Proc Natl Acad Sci U S A. 2000 May 23;97(11):6102-7. doi: 10.1073/pnas.110031497.
2
Recent advances in the study of prenylated proteins.异戊二烯化蛋白研究的最新进展。
Biochim Biophys Acta. 2000 Apr 12;1484(2-3):93-106. doi: 10.1016/s1388-1981(00)00009-3.
3
Regulatory networks controlling Candida albicans morphogenesis.控制白色念珠菌形态发生的调控网络。
Trends Microbiol. 1999 Aug;7(8):333-8. doi: 10.1016/s0966-842x(99)01556-5.
4
Dimorphism and virulence in Candida albicans.白色念珠菌的二态性与毒力
Curr Opin Microbiol. 1998 Dec;1(6):687-92. doi: 10.1016/s1369-5274(98)80116-1.
5
Flow sensing in mycelial fungi.丝状真菌中的流量感应
J Biotechnol. 1997 Dec 3;58(3):197-204. doi: 10.1016/s0168-1656(97)00147-8.
6
Derepressed hyphal growth and reduced virulence in a VH1 family-related protein phosphatase mutant of the human pathogen Candida albicans.人类病原体白色念珠菌的VH1家族相关蛋白磷酸酶突变体中菌丝生长去抑制和毒力降低。
Mol Biol Cell. 1997 Dec;8(12):2539-51. doi: 10.1091/mbc.8.12.2539.
7
Novel salvage pathway utilizing farnesol and geranylgeraniol for protein isoprenylation.利用法尼醇和香叶基香叶醇进行蛋白质异戊二烯化的新型补救途径。
Biochem Biophys Res Commun. 1997 Aug 28;237(3):483-7. doi: 10.1006/bbrc.1997.7145.
8
Nonfilamentous C. albicans mutants are avirulent.非丝状白色念珠菌突变体无致病性。
Cell. 1997 Sep 5;90(5):939-49. doi: 10.1016/s0092-8674(00)80358-x.
9
Control of filament formation in Candida albicans by the transcriptional repressor TUP1.转录阻遏物TUP1对白色念珠菌中丝状形成的调控
Science. 1997 Jul 4;277(5322):105-9. doi: 10.1126/science.277.5322.105.
10
Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi.Efg1p是人类病原体白色念珠菌形态发生的关键调节因子，属于一类保守的bHLH蛋白，这类蛋白在真菌中调节形态发生过程。
EMBO J. 1997 Apr 15;16(8):1982-91. doi: 10.1093/emboj/16.8.1982.
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