Fujimoto M, Kikuchi K, Tamaki T, Yazawa N, Kubo M, Ihn H, Sato S, Soma Y, Tamaki K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Br J Dermatol. 1997 Dec;137(6):916-20.
Anti-p80-coilin antibody produces a unique pattern of immunofluorescence staining called nuclear dots characterized by the presence of up to six discrete nuclear bodies in interphase cell nuclei. The distribution of this antibody and its clinical relevance have not been established. We studied the prevalence of anti-p80-coilin antibody in the sera from 810 patients with collagen diseases and various skin diseases. Five sera showed the nuclear dot pattern by indirect immunofluorescence on an HEp-2 cell substrate, and reacted by immunoblotting with 80-kDa protein in a nuclear extract from HeLa cells. Four of these sera were from patients with localized scleroderma, and the other was from a patient with primary Raynaud's phenomenon. The patients with localized scleroderma who were positive for anti-p80-coilin antibody had all been classified as having linear scleroderma. They had only one or two lesions, and were negative for antihistone and anti-single-stranded DNA antibodies. Our data indicate that anti-p80-coilin antibody is uncommon in skin diseases: however, this antibody is present in patients with a milder form of linear scleroderma, although the incidence of positivity may not be high.
抗p80卷曲螺旋蛋白抗体产生一种独特的免疫荧光染色模式，称为核点，其特征是在间期细胞核中存在多达六个离散的核体。这种抗体的分布及其临床相关性尚未明确。我们研究了810例胶原病和各种皮肤病患者血清中抗p80卷曲螺旋蛋白抗体的流行情况。5份血清在HEp-2细胞底物上通过间接免疫荧光显示出核点模式，并在免疫印迹中与来自HeLa细胞的核提取物中的80 kDa蛋白发生反应。其中4份血清来自局限性硬皮病患者，另一份来自原发性雷诺现象患者。抗p80卷曲螺旋蛋白抗体呈阳性的局限性硬皮病患者均被归类为线性硬皮病。他们只有一两个病灶，抗组蛋白和抗单链DNA抗体均为阴性。我们的数据表明，抗p80卷曲螺旋蛋白抗体在皮肤病中并不常见；然而，这种抗体存在于症状较轻的线性硬皮病患者中，尽管阳性发生率可能不高。
