School of Medical and Life Sciences, Escola de Ciências Médicas e da Vida, Pontifícia Universidade Católica de Goiás (PUC GOIÁS), Avenida Universitária 1.440, Setor Universitário, Goiânia, GO, 74605-010, Brazil.
Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
Adv Rheumatol. 2022 Sep 7;62(1):34. doi: 10.1186/s42358-022-00266-z.
The VI Brazilian Consensus on Autoantibodies against HEp-2 cells for determination of autoantibodies against cellular constituents on HEp-2 cells was held on September, 2019, in Fortaleza (CE, Brazil). The guidelines in this edition were formulated by the group of Brazilian experts discussing the classification of complex patterns, the classification of the nuclear discrete dots (few and multiple), the identification of the discrete fine speckled pattern (AC-4a) and improvements on the ANA report.
Sixteen Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of Brazil participated in the meeting. Four main topics were discussed: (1) How to classify patterns with fluorescence in more than one cell compartment considering three relevant categoris: composite patterns, mixed patterns and multiple patterns; (2) The splitting of the discrete nuclear dots pattern into the multiple discrete nuclear dots (AC-6) and few discrete nuclear dots (AC-7) patterns, respectively; (3) Inclusion of a novel nuclear pattern characterized by discrete fine speckled pattern highly associated with antibodies to SS-A/Ro60, classified as AC-4a. In addition, adjustments on the Brazilian Consensus nomenclature were implemented aiming to harmonize the designation of some patterns with the International Consensus on ANA Patterns (ICAP). Furthermore, the designations of the PCNA-like pattern (AC-13), CENP-F-like pattern (AC-14) and Topo I-like pattern (AC-29) were adjusted in accordance to ICAP. Finally, there was a recommendation for adjustment in the test report in order to address the status of nuclear envelope staining. For all topics, the aim was to establish specific guidelines for laboratories and clinicians. All recommendations were based on consensus among participants. All recommendations from the V Consensus were maintained and there was relevant progress in the BCA/HEp-2 guidelines and further harmonization with ICAP.
The VI BCA/HEp-2 edition was successful in establishing important recommendations regarding the classification of complex patterns, in supporting the identification of a novel pattern within the AC-4 group and in the harmonization process with the ICAP terminology.
2019 年 9 月，第六届巴西抗 HEp-2 细胞自身抗体共识会议在巴西福塔雷萨举行，旨在确定 HEp-2 细胞中细胞成分的自身抗体。本版指南由巴西专家组制定，讨论了复杂模式的分类、核离散点（少和多）的分类、离散细斑点模式（AC-4a）的识别以及 ANA 报告的改进。
16 名来自大学和临床实验室的巴西研究人员和专家代表巴西各地参加了会议。讨论了四个主要议题：（1）如何在超过一个细胞区室中对具有荧光的模式进行分类，考虑到三个相关类别：复合模式、混合模式和多模式；（2）将离散核点模式分别拆分为多个离散核点（AC-6）和少数离散核点（AC-7）模式；（3）纳入一种新的核模式，其特征为与 SS-A/Ro60 抗体高度相关的离散细斑点模式，归类为 AC-4a。此外，对巴西共识命名法进行了调整，旨在使一些模式的命名与国际抗核抗体模式共识（ICAP）协调一致。此外，PCNA 样模式（AC-13）、CENP-F 样模式（AC-14）和拓扑异构酶 I 样模式（AC-29）的命名也根据 ICAP 进行了调整。最后，建议调整检测报告，以说明核包膜染色的状态。所有议题的目的都是为实验室和临床医生制定具体的指南。所有建议均基于参与者的共识。第五届共识中的所有建议都得到了保留，BCA/HEp-2 指南取得了重要进展，并与 ICAP 进一步协调一致。
第六届巴西抗 HEp-2 细胞自身抗体共识会议成功制定了关于复杂模式分类的重要建议，支持在 AC-4 组内识别一种新的模式，并与 ICAP 术语保持一致。
