Lakey W H, Bruchovsky N, Callaway T, Comeau T, Lieskovsky G, Rennie P, Shnitka T, Wilkin P
Trans Am Assoc Genitourin Surg. 1979;71:19-22.
A new procedure has been developed to purify prostatic cell nuclei and to achieve a high yield of nuclear material for study. This technique allowed the investigation of the enzyme 5-alpha-reductase and its relationship to the translocation of dihydrotestosterone and androgen receptors into the nucleus of the prostatic cell. The studies revealed that 5-alpha-reductase was localized chiefly to the stromal components of all types of prostatic tissue. Although there is a higher concentration of dihydrotestosterone in carcinoma tissue the 5-alpha-reductase activity is low. Studies on the effect of androgens and antiandrogens on nuclear uptake of androgen revealed that androgens are potent stimulants of receptor translocation, while antiandrogens stimulate little or no translocation.
已开发出一种新方法来纯化前列腺细胞核，并获得用于研究的高产量核物质。该技术使得对5-α-还原酶及其与二氢睾酮和雄激素受体向前列腺细胞核内转运的关系进行研究成为可能。研究表明，5-α-还原酶主要定位于所有类型前列腺组织的基质成分中。虽然癌组织中二氢睾酮的浓度较高，但5-α-还原酶活性较低。关于雄激素和抗雄激素对雄激素核摄取影响的研究表明，雄激素是受体转运的有效刺激物，而抗雄激素对转运的刺激作用很小或没有。
