Chushkov P, Georgieva R, Gerov K
Vet Med Nauki. 1979;16(5):48-58.
A study on protein dynamics in calf blood serum from the time of birth to age of 60 days was carried out by use of electrophoresis on 1% agar gel, immunodiffusion and immunoelectrophoresis with rabbit immune serum against cattle blood serum, colostrum whey and pure nu-globulin. Investigations were performed on the 4th and 24th hour, and on the 3rd, 6th, 8th, 10th, 15th, 20th, 30th, 45th and 60th day after birth. Resorption of colostral gamma globulins, applied intravenously and orally in the form of the preparation lactoplasmin and 10% normal gamma-globulin injected intravenously was followed under experimental conditions. It was established that calves are born with a comparatively low protein level in the blood serum and after feeding with colostrum begins, specific quantitative and qualitative changes in their profile take place, but the changes concerning gamma-globulins are most pronounced. gamma-globulins are best resorbed after oral application of colostral globulin preparations, and not so well after intravenous application. Noral blood gamma-globulin injected intravenously is least resorbed. Eventual causes for this fact are discussed.
利用1%琼脂凝胶电泳、免疫扩散以及用兔抗牛血清、初乳乳清和纯丙种球蛋白免疫血清进行的免疫电泳,对出生至60日龄小牛血清中的蛋白质动态进行了研究。在出生后的第4小时和第24小时,以及第3天、第6天、第8天、第10天、第15天、第20天、第30天、第45天和第60天进行了调查。在实验条件下,追踪了以制剂乳铁蛋白和静脉注射10%正常丙种球蛋白的形式静脉内和口服给予的初乳丙种球蛋白的吸收情况。结果表明,小牛出生时血清中的蛋白质水平相对较低,开始喂初乳后,其蛋白质谱会发生特定的定量和定性变化,但与丙种球蛋白有关的变化最为明显。口服初乳球蛋白制剂后,丙种球蛋白的吸收效果最佳,静脉注射后的吸收效果则不太好。静脉注射正常血液丙种球蛋白的吸收效果最差。文中讨论了这一事实的可能原因。