Connaughton V P, Dowling J E
Marine Biological Laboratory, MA 02543, USA.
Vision Res. 1998 Jan;38(1):13-8. doi: 10.1016/s0042-6989(97)00146-6.
Distal retinal cells from larval (7-10 days postfertilization) and adult zebrafish retinas were cultured in 70% L-15 medium for 4-5 d and comparable cell types identified. Four photoreceptor types were observed in adult retinal cultures, whereas only single cones were isolated from larval retinas. Horizontal cells in both larval and adult cultures were distinguished by their large size and stellate morphology and two subtypes, A and B, were recognized. Bipolar cells were readily identified in adult cultures, but rare in larval cultures. Two bipolar cell types, large and small, were distinguished. Measurements of the various cell types are provided.
将来自幼虫期(受精后7 - 10天)和成年斑马鱼视网膜的远端视网膜细胞在70%的L - 15培养基中培养4 - 5天,并鉴定出可比的细胞类型。在成年视网膜培养物中观察到四种光感受器类型,而从幼虫视网膜中仅分离出单个视锥细胞。幼虫和成年培养物中的水平细胞以其大尺寸和星状形态为特征,可识别出两种亚型,A和B。双极细胞在成年培养物中很容易识别,但在幼虫培养物中很少见。区分出两种双极细胞类型,大的和小的。提供了各种细胞类型的测量数据。