Tokugawa Y, Kunishige I, Kubota Y, Shimoya K, Nobunaga T, Kimura T, Saji F, Murata Y, Eguchi N, Oda H, Urade Y, Hayaishi O
Department of Obstetrics and Gynecology, Osaka University Medical School, Suita, Japan.
Biol Reprod. 1998 Feb;58(2):600-7. doi: 10.1095/biolreprod58.2.600.
Prostaglandin D synthase (PGDS) activity was detected in human seminal plasma (0.05-1.83 nmol/min per milligram protein). The enzyme was purified from human seminal plasma by immunoaffinity chromatography and found to be 27 kDa in size and N-glycosylated, similar to PGDS in the cerebrospinal fluid. The N-terminal amino acid sequence of 16 residues of the seminal enzyme, APEAQVSVQPNFQQDK, was identical to that of the cerebrospinal fluid PGDS. Although PGDS activity and the content determined by the immunoassay each highly varied in the seminal plasma, the concentration was significantly (p < 0.001) lower in the oligozoospermic group (2.47 +/- 0.51 microg/ml) than in the normozoospermic group (9.75 +/- 1.49 microg/ml). Prostaglandin (PG) D2 was detected in the seminal plasma (5.00 +/- 0.65 ng/ml) with a positive correlation to the PGDS concentration (p < 0.05). PGD2 was converted to the J series of PGs in the seminal plasma with a half-life of 6.5 h. Northern blot analysis revealed that mRNA for PGDS was expressed in the testis, prostate, and epididymis. Through immunohistochemistry, PGDS was localized in Leydig cells of the testis and in epithelial cells of the prostate and ductus epididymidis.
在人精浆中检测到前列腺素 D 合酶（PGDS）活性（每毫克蛋白质 0.05 - 1.83 纳摩尔/分钟）。通过免疫亲和层析从人精浆中纯化该酶，发现其大小为 27 kDa 且为 N - 糖基化，与脑脊液中的 PGDS 相似。精浆中该酶 16 个残基的 N 端氨基酸序列 APEAQVSVQPNFQQDK 与脑脊液 PGDS 的相同。尽管精浆中 PGDS 活性和免疫测定所确定的含量各自变化很大，但少精子症组（2.47 ± 0.51 微克/毫升）中的浓度显著（p < 0.001）低于正常精子症组（9.75 ± 1.49 微克/毫升）。在精浆中检测到前列腺素（PG）D2（5.00 ± 0.65 纳克/毫升），其与 PGDS 浓度呈正相关（p < 0.05）。PGD2 在精浆中转化为 J 系列前列腺素，半衰期为 6.5 小时。Northern 印迹分析显示，PGDS 的 mRNA 在睾丸、前列腺和附睾中表达。通过免疫组织化学，PGDS 定位于睾丸的 Leydig 细胞以及前列腺和附睾管的上皮细胞中。
