无精子症精液中脂质运载蛋白型前列腺素D合成酶与α-葡萄糖苷酶的关系
Relationship between lipocalin-type prostaglandin D synthase and alpha-glucosidase in azoospermia seminal plasma.
作者信息
Chen De-Yu, Wang Jun-Jun, Huang Yu-Feng, Zhou Kai-Ya
机构信息
College of Life Science, Nanjing Normal University, Nanjing, China; Laboratory of Reproduction and Genetics, JinLing Hospital, Nanjing, China.
出版信息
Clin Chim Acta. 2005 Apr;354(1-2):69-76. doi: 10.1016/j.cccn.2004.11.009. Epub 2004 Dec 30.
BACKGROUND
[corrected] To determine the correlation of lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase in semen.
METHODS
We analyzed 68 seminal plasmas for lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase, L-PGDS was analyzed by ELISA. The semen donors were categorized in 3 groups: normal, obstructive and non-obstructive azoospermia. We then evaluated their correlation.
RESULTS
The difference of L-PGDS concentration (P<0.001) and alpha-glucosidase activity (P<0.001) among the 3 clinical groups was statistically significant. Correlation between L-PGDS concentration and alpha-glucosidase was also statistically significant. L-PGDS concentration correlated positively with alpha-glucosidase activity (r=0.882).
CONCLUSIONS
L-PGDS in seminal plasma, like alpha-glucosidase, suggests an obstruction of the seminal ducts and may be a potential marker that may aid in the differential diagnosis of obstructive and non-obstructive azoospermia.
背景
[已校正] 确定精液中脂质运载蛋白型前列腺素D合成酶（L-PGDS）与α-葡萄糖苷酶之间的相关性。
方法
我们分析了68份精液中的脂质运载蛋白型前列腺素D合成酶（L-PGDS）和α-葡萄糖苷酶，L-PGDS采用酶联免疫吸附测定法进行分析。精液捐献者被分为3组：正常、梗阻性和非梗阻性无精子症。然后我们评估了它们之间的相关性。
结果
3个临床组之间L-PGDS浓度（P<0.001）和α-葡萄糖苷酶活性（P<0.001）的差异具有统计学意义。L-PGDS浓度与α-葡萄糖苷酶之间的相关性也具有统计学意义。L-PGDS浓度与α-葡萄糖苷酶活性呈正相关（r=0.882）。
结论
精液中的L-PGDS与α-葡萄糖苷酶一样，提示存在输精管道梗阻，可能是有助于梗阻性和非梗阻性无精子症鉴别诊断的潜在标志物。
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