Regena（Rga）是酵母中全局负转录调节因子CDC36（NOT2）的果蝇同源物，它可修饰基因表达并抑制位置效应斑驳。
Regena (Rga), a Drosophila homolog of the global negative transcriptional regulator CDC36 (NOT2) from yeast, modifies gene expression and suppresses position effect variegation.
作者信息
Frolov M V, Benevolenskaya E V, Birchler J A
机构信息
Division of Biological Sciences, University of Missouri-Columbia 65211, USA.
出版信息
Genetics. 1998 Jan;148(1):317-29. doi: 10.1093/genetics/148.1.317.
Abstract
A mutation in Regena (Rga) was isolated in screens for modifiers of white eye color gene expression. The reduction in the level of the Rga product results in a complex modulation of white mRNA both positively and negatively, depending on the developmental stage. In addition to white, Rga also affects the expression of several other tested genes, with one of them, Vinculin, being regulated in a strong sex-specific manner. Rga was cloned by transposon tagging. Its predicted product lacks any recognized nucleic acid-binding motif but is homologous to a global negative transcriptional regulator, CDC36 (NOT2), from yeast. Rga also acts as a suppressor of position effect variegation, suggesting that a possible function of Rga could be mediation of an interaction between chromatin proteins and the transcriptional complex.
摘要
在筛选白眼颜色基因表达修饰因子的过程中分离出了Regena（Rga）的一个突变体。Rga产物水平的降低会根据发育阶段对白色mRNA产生正向和负向的复杂调节。除了白色基因外，Rga还影响其他几个测试基因的表达，其中之一的纽蛋白（Vinculin）以强烈的性别特异性方式受到调控。Rga通过转座子标签法克隆得到。其预测产物缺乏任何公认的核酸结合基序，但与酵母中的一种全局负转录调节因子CDC36（NOT2）同源。Rga还作为位置效应斑驳的抑制因子起作用，这表明Rga的一个可能功能可能是介导染色质蛋白与转录复合物之间的相互作用。
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